The Transgenic Core generates transgenic and gene knockout mouse models relevant to obesity, diabetes and nutrition research. The Core also provides consultation on all aspects of making transgenic and gene knockout mice.

Activities

• Generation of Transgenic Mice. DNA transgenic constructs, supplied by the investigator, are microinjected into fertilized one cell embryos. Tails from potentially transgenic founders are then supplied to the investigator for genetic analysis. Identified founders are then provided to the investigator.
• Gene Targeting - Generation of Targeted ES cells. DNA gene knockout constructs, supplied by the investigator, are electroporated into W4/129S6 embryonic stem cells. Drug resistant clones are selected, expanded and frozen down for subsequent use. Aliquots of cells from each clone are provided to the investigator for genetic identification of targeted clones.
• Gene Targeting - Generation of Chimeric Mice from Targeted ES Cells. Targeted ES cells are injected into 3 day old embryos (blastocysts). Highly chimeric mice are then provided to the investigator.
• Cryopreservation of Mutant Mice. Fertilized once cell embryos are cryopreserved (contact Transgenic Core for details).
• Consultation. Consultation on all aspects of transgenic and gene knockout work is provided.